Report
Health Monitoring in Accordance with FELASA Recommendations

Date of issue:23 May 2018
Species:mice (exclusively)
Housing:SPF, Barrier IVC/1.

Test FrequencyLatest Test DateNumberLatest resultTesting Laborat.Test MethodHistorical result (<18 months)
 
Viruses 
MHV (Mouse hepatitis virus)3 months22 Jan 20180/7negative1IFA0/31
EDIM (mouse ROTA)3 months22 Jan 20180/7negative1IFA0/31
MVM, (NS1*)3 months22 Jan 20180/7negative1PCR0/31
MPV 1+2, (NS1*)3 months22 Jan 20180/7negative1PCR0/31
Theiler (TMEV)3 months22 Jan 20180/7negative1IFA0/31
MNV (Mouse norovirus)3 months22 Jan 20180/7negative1IFA0/31
PVMAnnually22 Jan 20180/7negative1IFA0/26
SENDAnnually22 Jan 20180/7negative1IFA0/26
ECTRO (Ectromelia virus)Annually22 Jan 20180/7negative1IFA0/26
LCMV (Lymphocytic choriomeningitis virus)Annually22 Jan 20180/7negative1IFA0/26
MAD (Mouse adenovirus) 1+2Annually22 Jan 20180/7negative1IFA0/26
REO-3 (Reovirus 3)Annually22 Jan 20180/7negative1IFA0/26
 
Bacteria, Mycoplasma and fungi 
Pasteurella pneumotropica3 months22 Jan 20180/7negative1IFA0/36
Streptococci Beta-haemolytic (not group D)3 months25 Jan 20180/7negative2CULT0/31
Streptococcus pneumoniae3 months25 Jan 20180/7negative2CULT0/31
Helicobacter spp.3 months22 Jan 20180/7negative1PCR0/31
CPIL (Clostridium piliforme)Annually22 Jan 20180/7negative1IFA0/26
Citrobacter rodentiumAnnually25 Jan 20180/7negative2CULT0/26
Corynebacterium kutscheriAnnually25 Jan 20180/7negative2CULT0/26
Mycoplasma pneumoniaeAnnually22 Jan 20180/7negative1IFA0/26
Salmonella spp.Annually25 Jan 20180/7negative2CULT0/26
Streptobacillus moniliformisAnnually25 Jan 20180/7negative2CULT0/26
 
Ecto parasites 
Mites3 months25 Jan 20180/7negative2MICR0/31
Lices3 months25 Jan 20180/7negative2MICR0/31
 
Endo parasites 
Aspiculuris tetraptera3 months25 Jan 20180/7negative2MICR0/31
Syphacia muris3 months25 Jan 20180/7negative2MICR0/31
Syphacia obvelata3 months25 Jan 20180/7negative2MICR0/31
Entamoeba muris3 months25 Jan 20180/7negative2MICR1/31
Giardia sp.3 months25 Jan 20180/7negative2MICR0/31
Spironucleus sp.3 months25 Jan 20180/7negative2MICR0/31
Trichomonads3 months25 Jan 20180/7negative2MICR0/31
 
Non pathogen endoparasites 
Hexamastix sp.3 months25 Jan 20180/7negative2MICR2/31
Chilomastix sp.3 months25 Jan 20180/7negative2MICR0/31
 
Pathological lesions 
External3 months25 Jan 20180/7negative2PATH/HIST0/31
Necropsy3 months25 Jan 20180/7negative2PATH/HIST0/31

 

Data are expressed as number positive/number tested

 

Abbreviation used in this report:

 

ELISA  = enzyme linked immunosorbent assay, MICR = microscopy, IFA = immunoflorescence assay, CULT = culture, PATH = gross pathology, PCR = polymerase chain reaction, HIST = histopathology, NT = not tested

 

 

Diagnostic laboratories:

1/ BioDoc LTD, Feodor Lynen str. 23, 30625 Hannover, Germany

2/ Central Veterinary Institute, Tábornok u. 2, 1149 Budapest, Hungary.

 

Remarks:

 

The sentinel program consists of at least quarterly necropsy, serology, and parasitology following the recommendation of FELASA. These sentinels are housed on a sample of dirty bedding from the other animals’ cages from a given room.

* NS1 = NS1 protein is highly conserved among rodent parvoviruses and thus serves as a generic assay for parvovirus seroconversion.

 

The ‘Serology/Parasitology History’ provided above indicates previous sentinel results and is not a guarantee of the current or future health status; it is only an indicator of the previous health status. A conventional animal house is running in the same building with MHV and pinworms.

 

This document has been changed conforming the new recommendations made by the FELASA working group on revision of guidelines for health monitoring of rodents and rabbits in February, 2014. 

http://lan.sagepub.com/content/early/2014/02/18/0023677213516312

 

 

 

 

                                                                                                                       

 

                                                                                                                        Balazs Benyei               

 


1094. Budapest, Tűzoltó utca 37/47, Hungary

Tel: +36-1-4591500/60390, mobil: +36-20-6632833

Fax: +36-1-4591487

Email: balazs.benyei@semmelweis-univ.hu