Report
Health Monitoring in Accordance with FELASA Recommendations

Date of issue:11 July 2020
Species:mice (exclusively)
Housing:SPF, Barrier IVC/1.

Test FrequencyLatest Test DateNumberLatest resultTesting Laborat.Test MethodHistorical result (<18 months)
 
Viruses 
MHV (Mouse hepatitis virus)3 months18 June 20200/16negative1IFA0/78
EDIM (mouse ROTA)3 months18 June 20200/16negative1IFA0/78
MVM, (NS1*)3 months18 June 20200/16negative1PCR0/78
MPV 1+2, (NS1*)3 months18 June 20200/16negative1PCR0/78
Theiler (TMEV)3 months18 June 20200/16negative1IFA0/78
MNV (Mouse norovirus)3 months18 June 20200/16negative1IFA0/78
PVMAnnually16 Aug 20190/15negative1IFA0/15
SENDAnnually16 Aug 20190/15negative1IFA0/15
ECTRO (Ectromelia virus)Annually16 Aug 20190/15negative1IFA0/15
LCMV (Lymphocytic choriomeningitis virus)Annually16 Aug 20190/15negative1IFA0/15
MAD (Mouse adenovirus) 1+2Annually16 Aug 20190/16negative1IFA0/16
REO-3 (Reovirus 3)Annually16 Aug 20190/16negative1IFA0/16
 
Bacteria, Mycoplasma and fungi 
Pasteurella pneumotropica3 months18 June 20200/16negative1IFA0/78
Streptococci Beta-haemolytic (not group D)3 months30 Mar 20200/16negative2CULT0/62
Streptococcus pneumoniae3 months30 Mar 20200/16negative2CULT0/62
Helicobacter spp.3 months18 June 20200/16negative1PCR0/78
CPIL (Clostridium piliforme)Annually16 Aug 20190/15negative1IFA0/15
Citrobacter rodentiumAnnually20 Nov 20190/16negative2CULT0/16
Corynebacterium kutscheriAnnually20 Nov 20190/16negative2CULT0/16
Mycoplasma pneumoniaeAnnually16 Aug 20190/15negative1IFA0/15
Salmonella spp.Annually20 Nov 20190/16negative2CULT0/16
Streptobacillus moniliformisAnnually20 Nov 20190/16negative2CULT0/31
 
Ecto parasites 
Mites3 months30 Mar 20200/16negative2MICR0/62
Lices3 months30 Mar 20200/16negative2MICR0/62
 
Endo parasites 
Aspiculuris tetraptera3 months30 Mar 20200/16negative2MICR0/62
Syphacia muris3 months30 Mar 20200/16negative2MICR0/62
Syphacia obvelata3 months30 Mar 20200/16negative2MICR0/62
Entamoeba muris3 months30 Mar 20200/16negative2MICR0/62
Giardia sp.3 months30 Mar 20200/16negative2MICR0/62
Spironucleus sp.3 months30 Mar 20200/16negative2MICR0/62
Trichomonads3 months30 Mar 20200/16negative2MICR0/62
 
Non pathogen endoparasites 
Hexamastix sp.3 months30 Mar 20200/16negative2MICR0/62
Chilomastix sp.3 months30 Mar 20200/16negative2MICR0/62
 
Pathological lesions 
External3 months30 Mar 20200/16negative2PATH/HIST0/62
Necropsy3 months30 Mar 20200/16negative2PATH/HIST0/62

 

Data are expressed as number positive/number tested

 

Abbreviation used in this report:

 

ELISA  = enzyme linked immunosorbent assay, MICR = microscopy, IFA = immunoflorescence assay, CULT = culture, PATH = gross pathology, PCR = polymerase chain reaction, HIST = histopathology, NT = not tested

 

 

Diagnostic laboratories:

1/ BioDoc LTD, Feodor Lynen str. 23, 30625 Hannover, Germany

2/ Central Veterinary Institute, Tábornok u. 2, 1149 Budapest, Hungary.

 

Remarks:

 

The sentinel program consists of at least quarterly necropsy, serology, and parasitology following the recommendation of FELASA. These sentinels are housed on a sample of dirty bedding from the other animals’ cages from a given room.

* NS1 = NS1 protein is highly conserved among rodent parvoviruses and thus serves as a generic assay for parvovirus seroconversion.

 

The ‘Serology/Parasitology History’ provided above indicates previous sentinel results and is not a guarantee of the current or future health status; it is only an indicator of the previous health status. A conventional animal house is running in the same building with MHV and pinworms.

 

This document has been changed conforming the new recommendations made by the FELASA working group on revision of guidelines for health monitoring of rodents and rabbits in February, 2014. 

http://lan.sagepub.com/content/early/2014/02/18/0023677213516312

 

 

 

 

                                                                                                                       

 

                                                                                                                        Balazs Benyei               

 


1094. Budapest, Tűzoltó utca 37/47, Hungary

Tel: +36-1-4591500/60390, mobil: +36-20-6632833

Fax: +36-1-4591487

Email: balazs.benyei@semmelweis-univ.hu